abstract：:In this study, we determined the induction and time-dependent accumulation of micronuclei in the peripheral blood of transgenic C57BL/6 p53+/- mice (p53+/- mice), FVB/N Tg.AC v-Ha-ras mice (Tg.AC mice) and their isogenic parental strains, FVB/N and C57BL/6 following inhalation exposure to benzene. Our objective was to...

journal_title：Mutagenesis

authors： Healy LN,Pluta LJ,James RA,Janszen DB,Torous D,French JE,Recio L

更新日期：2001-03-01 00:00:00

abstract：:The adduct that would arise from cis opening of (+)-(1S,2R,3R, 4S)-3,4-dihydroxy-1,2-epoxy-benzo[c]phenan-threne (benzo[c]phenanthrene diol epoxide-2, where the benzylic hydroxyl group and the epoxide oxygen are trans) by the exocyclic N6-amino group of deoxyadenosine was incorporated at the marked site into four olig...

journal_title：Mutagenesis

authors： Pontén I,Waters LS,Sayer JM,Pilcher AS,Dipple A,Jerina DM

更新日期：2001-01-01 00:00:00

abstract：:To evaluate the genetic damage induced by tuberculosis infection and also by a combination of therapeutic exposure to anti-tuberculosis (anti-TB) drugs (isoniazid + rifampicin + pyrazinamide + ethambutol or streptomycin) we have considered chromosome aberrations (CA) and micronuclei (MN) in binucleate peripheral blood...

journal_title：Mutagenesis

authors： Masjedi MR,Heidary A,Mohammadi F,Velayati AA,Dokouhaki P

更新日期：2000-11-01 00:00:00

abstract：:Each of the Escherichia coli tester strains in the WP3101P-WP3106P series contains an F' plasmid with a different base substitution mutation within the lacZ gene. Each of the six possible base substitution mutations, therefore, can be assayed with these strains by Lac(+) reversion. We used the strains to characterize ...

journal_title：Mutagenesis

authors： Ohta T,Watanabe-Akanuma M,Yamagata H

更新日期：2000-07-01 00:00:00

abstract：:The genotoxicity of atenolol, a beta-blocker antihypertensive drug, both in vitro and in vivo, was cytogenetically tested for its ability to induce sister chromatid exchange (SCE) and micronuclei (MN) in cultured peripheral lymphocytes. Also, fluorescence in situ hybridization (FISH) with a centromeric probe was perfo...

journal_title：Mutagenesis

authors： Télez M,Martínez B,Criado B,Lostao CM,Penagarikano O,Ortega B,Flores P,Ortiz-Lastra E,Alonso RM,Jiménez RM,Arrieta I

更新日期：2000-05-01 00:00:00

abstract：:We carried out statistical analysis of the frequency of loss of heterozygosity (LOH) at 10 microsatellite markers on chromosome 9. In 44 microdissected sporadic primary melanomas a comparison of LOH frequency data with other patient data, like age at diagnosis and tumour thickness, showed an interesting correlation be...

journal_title：Mutagenesis

authors： Smeds J,Kumar R,Rozell BL,Hemminki K

更新日期：2000-05-01 00:00:00

abstract：:Primary liver fibroblasts were applied in a cytokinesis-block micronucleus assay in combination with fluorescence in situ hybridization (FISH) using two protocols. In protocol A (Prot. A), cytochalasin B (Cyt B) was added at the end of the treatment time directly to the medium containing the standard compounds, wherea...

journal_title：Mutagenesis

authors： Nesti C,Trippi F,Scarpato R,Migliore L,Turchi G

更新日期：2000-03-01 00:00:00

abstract：:Sanfetrinem cilexetil, a hexetil ester of a Trinem antibiotic, does not induce micronuclei in rat bone marrow cells or induce DNA repair synthesis in rat hepatocytes following oral dosing. However, in vitro chromosome damage and mutations are induced in mammalian cells lacking carboxylesterase activity (human lymphocy...

journal_title：Mutagenesis

authors： Oliver J,Naidoo A,Vandin L,Pugnaghi F,Gatehouse D,Comelli R

更新日期：2000-01-01 00:00:00

abstract：:The aim of the present work was to investigate the processes involved in the origin of trisomic karyotypes, i.e. co-migration of sister chromatids (mitotic non-disjunction, MND) and recovery of micronuclei (MN) originating from lagging chromosomes/chromatids at anaphase (mitotic indirect non-disjunction, MIND), and to...

journal_title：Mutagenesis

authors： Minissi S,Degrassi F,Tanzarella C,Gustavino B

更新日期：1999-11-01 00:00:00

abstract：:On April 6, 1993, near the town of Tomsk (Russia) there was an accident at the Siberian Chemical Plant (SCP) which resulted in extensive contamination of an area of 250 km(2) to the north of SCP with long-lived radionuclides such as (239)Pu, (137)Cs and (90)Sr. Cytogenetic methods and electron spin resonance (ESR) spe...

journal_title：Mutagenesis

authors： Ilyinskikh NN,Ilyinskikh IN,Porovskiy VA,Natarajan AT,Suskov II,Smirenniy LN,Ilyinskikh EN

更新日期：1999-09-01 00:00:00

abstract：:AHH-1 TK+/- is a human B cell-derived lymphoblastoid cell line that constitutively expresses a high level of the cytochrome CYP1A1. The MCL-5 cell line was developed by transfection of AHH-1 with cDNAs encoding the human cytochrome P450s, CYP1A2, CYP2A6, CYP2E1, CYP3A4 and microsomal epoxide hydrolase carried in plasm...

journal_title：Mutagenesis

authors： Corso C,Parry EM

更新日期：1999-07-01 00:00:00

abstract：:With the aim of optimizing the efficiency of S9 fractions used in in vitro mutagenicity assays, different schemes for the induction of liver enzymes in rats were tried and the amount of S9 fraction required was assessed. The activity of 2-anthramine (2AA), 2-acetylaminofluorene (2AAF), 3-methylcholanthrene (3MTCL) and...

journal_title：Mutagenesis

authors： García Franco S,Domínguez G,Pico JC

更新日期：1999-05-01 00:00:00

abstract：:The use of a white-ivory (wi) strain of Drosophila melanogaster carrying four copies of this allele, (wi)4, has proved to be useful in detecting somatic mutation in genotoxicity testing. Nevertheless, until now very little information exists about the nature of the genetic effects detected in such a strain. This work ...

journal_title：Mutagenesis

authors： Suárez S,Cabré O,Velázquez A,Marcos R,Xamena N

更新日期：1999-03-01 00:00:00

abstract：:The spectrum of mutations induced by cobalt(II) chloride (CoCl2) was examined using plasmid pUB3 DNA, which was propagated after transfection into Escherichia coli SY1032/pKY241 host cells. The vector plasmid carried an E.coli supF suppressor tRNA gene as a target for mutations. After CoCl2 treatment, 64 independent n...

journal_title：Mutagenesis

authors： Ogawa HI,Ohyama Y,Ohsumi Y,Kakimoto K,Kato Y,Shirai Y,Nunoshiba T,Yamamoto K

更新日期：1999-03-01 00:00:00

abstract：:The aim of the present work was to investigate the possible interference of cytochalasin B (cyt B) with low concentration treatment with colchicine in the induction of chromosome/chromatid loss and micronuclei in human lymphocytes mitotically activated in vitro. Thus, cells from a single female donor were treated with...

journal_title：Mutagenesis

authors： Minissi S,Gustavino B,Degrassi F,Tanzarella C,Rizzoni M

更新日期：1999-01-01 00:00:00

abstract：:In the present study we evaluated campthotecin (CPT)-induced chromosomal damage in human lymphocytes in the G0 phase of the cell cycle as revealed by the premature chromosome condensation technique. The results obtained here indicate that CPT was able to induce chromosome fragments in the G0 phase of the cell cycle of...

journal_title：Mutagenesis

authors： Mosesso P,Fonti E,Bassi L,Lorenti Garcia C,Palitti F

更新日期：1999-01-01 00:00:00

abstract：:The XPF and ERCC1 proteins form a tight complex and function as an endonuclease to incise on the 5'-side of pyrimidine dimers in DNA. Levels of both proteins are extremely low in group F xeroderma pigmentosum (XP-F) cells. We transfected XP-F cells with the plasmids expressing XPF or ERCC1 and examined levels of both ...

journal_title：Mutagenesis

authors： Yagi T,Katsuya A,Koyano A,Takebe H

更新日期：1998-11-01 00:00:00

abstract：:The genotoxic effects of benzene in lung cells of mice exposed to single acute doses by inhalation have been estimated by cytogenetic analysis of micronuclei in primary cultures of lung fibroblasts. Mice were nose-only exposed to 1000 p.p.m. for 30 or 60 min or to 3500 p.p.m. for 30 min and sacrificed 24 h after the e...

journal_title：Mutagenesis

authors： Ranaldi R,Bassani B,Villani P,Lombardi CC,Tanzarella C,Pacchierotti F

更新日期：1998-09-01 00:00:00

abstract：:Carbaryl causes depolymerization of spindle microtubules and apparent uncoupling of karyokinesis and cytokinesis in mitotic V79 cells. The metabolite 1-naphthol has virtually identical effects at equimolar concentrations. The closely related 2-naphthol causes similar configurations, but at a much lower frequency than ...

journal_title：Mutagenesis

authors： Renglin A,Olsson A,Wachtmeister CA,Onfelt A

更新日期：1998-07-01 00:00:00

abstract：:Colcemid (COM) and econazole (EZ) were tested for induction of mitotic arrest and C-mitotic effects in mouse bone marrow cells and for meiotic delay and induction of hyperploidy in mouse spermatocytes after single injection. Doses of 1 and 3 mg/kg COM were used and bone marrow and spermatocytes were sampled at 2, 6, 1...

journal_title：Mutagenesis

authors： Shi Q,Adler ID

更新日期：1998-07-01 00:00:00

abstract：:Aneuploidy has important consequences for human health. There is a requirement to detect aneugens that may not be fully satisfied by the current test batter. New cytogenetic techniques look promising. ...

journal_title：Mutagenesis

authors：

更新日期：1998-07-01 00:00:00

abstract：:Non-cytotoxic concentrations (1.5, 3 and 6 microM) of sodium arsenite (SA) were used to study its cytogenetic effects with special reference to the frequency and nature of chromosomal aberrations on the X chromosome at Xq21 and at centromeric regions in CHO9, EM-C11, V79, V-H4 and CHE cell lines using Giemsa and FISH ...

journal_title：Mutagenesis

authors： Radha S,Natarajan AT

更新日期：1998-05-01 00:00:00

abstract：:This is an investigation of the long-term effects of a shift from a mixed diet to a lacto-vegetarian diet and of a return to a mixed diet on the mutagenic activity in urine and faeces. The participants were 20 normal weight, non-smoking subjects. Dietary surveys and urinary and faecal samples were collected before and...

journal_title：Mutagenesis

authors： Johansson G,Holmén A,Persson L,Högstedt B,Wassén C,Ottova L,Gustafsson JA

更新日期：1998-03-01 00:00:00

abstract：:Macrocyclic polyethers (crown ethers) are a family of compounds that possess the ability to complex with and transport metal ions across membranes. Because of their unique ionophoric characteristic, they have wide application in industry and research, chemistry and biology. In the current investigation the relationshi...

journal_title：Mutagenesis

authors： Cai MY,Arenaz P

更新日期：1998-01-01 00:00:00

abstract：:Hyperbaric oxygen (HBO) treatment as used therapeutically has been shown to induce DNA damage in the alkaline comet assay with leukocytes from test subjects. Using formamidopyrimidine-DNA glycosylase, a DNA repair enzyme which specifically nicks DNA at sites of 8-oxoguanines and formamidopyrimidines, we have detected ...

journal_title：Mutagenesis

authors： Speit G,Dennog C,Lampl L

更新日期：1998-01-01 00:00:00

abstract：:The adaptive response is a phenomenon by which cells exposed to low, non-cytotoxic doses of a genotoxicant become significantly resistant to a subsequent higher dose of the same or another genotoxic agent. Induction of the adaptive response has been mainly studied using ionizing radiation and alkylating agents as geno...

journal_title：Mutagenesis

authors： Oliveira NG,Rodrigues AS,Chaveca T,Rueff J

更新日期：1997-11-01 00:00:00

abstract：:We employed the three-way differential staining technique (TWD), which allows SCEs to be distinguished on a per generation basis by scoring third metaphases (M3), in order to study the spontaneous levels of SCEs in normal and high frequency cells (HFCs) that occurred in the first (S1), second (S2) and third (S3) S pha...

journal_title：Mutagenesis

authors： Ponzanelli I,Landi S,Bernacchi F,Barale R

更新日期：1997-09-01 00:00:00

abstract：:We have characterized six SV40-transformed xeroderma pigmentosum cell lines (XP20S and XP12BE derived from female donors, XP12RO-SV, XP3BR/12SV, XP4PA-SV and XP8CAC-SV from male donors) for their usability in HPRT mutation studies. All cell lines exhibit hypersensitivity, compared with MRC5CV1 cells, towards the cytot...

journal_title：Mutagenesis

authors： Merk O,Speit G

更新日期：1997-09-01 00:00:00

abstract：:CI Solvent Yellow 14 has been reported to be carcinogenic in the rat, inducing neoplastic liver nodules, but non-carcinogenic in the mouse. The present experiments have extended previously reported investigations on the activity of CI Solvent Yellow 14 in in vivo genotoxicity assays. CI Solvent Yellow 14 has been exam...

journal_title：Mutagenesis

authors： Elliott BM,Griffiths K,Mackay JM,Wade JD

更新日期：1997-07-01 00:00:00

abstract：:In order to establish the capacity of Mytilus spp. to form genotoxin-DNA adducts, a series of in vitro and in vivo studies were conducted in which tissue samples and animals were exposed to five model genotoxins. Following the in vitro characterization of the major adducts induced by the compounds, a series of in vivo...

journal_title：Mutagenesis

authors： Harvey JS,Parry JM

更新日期：1997-05-01 00:00:00

abstract：:Quantitative and qualitative aspects of the in vivo micronucleus-inducing potential of five chemicals were studied using flow cytometric enumeration of micronucleated polychromatic peripheral blood erythrocytes in mice. The chemicals were hydroquinone, vinblastine sulphate, chloral hydrate (tested in two different mou...

journal_title：Mutagenesis

authors： Grawé J,Nüsse M,Adler ID

更新日期：1997-01-01 00:00:00

abstract：:The present study was undertaken to compare the antimutagenic activity of aqueous extracts, at the concentrations used for human consumption, from green, black and decaffeinated black tea. Antimutagenic potential was evaluated against three indirect-acting dietary carcinogens, Glu-P-1, benzo(a)pyrene and nitrosopyrrol...

journal_title：Mutagenesis

authors： Bu-Abbas A,Nunez X,Clifford MN,Walker R,Ioannides C

更新日期：1996-11-01 00:00:00

abstract：:Polychlorinated biphenyls (PCBs) are stable pollutants, which can be found in almost every compartment of terrestrial and aquatic ecosystems. They are very lipophilic and therefore have the potency of accumulating in the fat stores of animals. The mechanisms by which PCBs exert their adverse effects are still unclear....

journal_title：Mutagenesis

authors： Belpaeme K,Delbeke K,Zhu L,Kirsch-Volders M

更新日期：1996-09-01 00:00:00

abstract：:The antineoplastic drug etoposide (ET) inhibits topoisomerase II (topo II) activity by forming a ternary complex (DNA-ET-topo II). This complex prevents the DNA-strand-rejoining activity of topo II and may result in structural chromosome aberrations. Inhibition of topo II activity may also predispose cells to aneuploi...

journal_title：Mutagenesis

authors： Mailhes JB,Marchetti F,Young D,London SN

更新日期：1996-07-01 00:00:00

abstract：:The human lymphocyte micronucleus (MN) test combined with fluorescence in situ hybridization (FISH) of a centromeric probe is considered a useful screening assay to distinguish between clastogenic and aneugenic agents. Four suspected aneuploidy-inducing chemicals, acetaldehyde (AA), diethylstilbestrol (DES), diethylst...

journal_title：Mutagenesis

authors： Migliore L,Cocchi L,Scarpato R

更新日期：1996-05-01 00:00:00

abstract：:Adducts of 1,2-epoxy-3-butene (EB) with haemoglobin were monitored in 17 workers from the 1,3-butadiene (BD) production unit of a petrochemical plant and in nine referents employed at the same factory but not exposed to BD. The air concentrations of BD were determined using stationary and personal monitoring. The ambi...

journal_title：Mutagenesis

authors： Osterman-Golkar S,Peltonen K,Anttinen-Klemetti T,Landin HH,Zorcec V,Sorsa M

更新日期：1996-03-01 00:00:00

abstract：:Urine samples from 26 cigarette smokers on a restricted diet were collected in the late afternoon. Urine extracts on XAD-2 resin were tested for mutagenicity in the microsuspension assay using Salmonella typhimurium strain TA98 in the presence of metabolizing and deconjugating enzymes. Levels of urinary nicotine plus ...

journal_title：Mutagenesis

authors： Granella M,Priante E,Nardini B,Bono R,Clonfero E

更新日期：1996-03-01 00:00:00

abstract：:We have developed a two dose/single-sample protocol for the in vivo/in vitro rat hepatocyte unscheduled DNA synthesis (UDS) assay. In order to enhance the effectiveness of grain detection by image analysis we found minor technical modifications to be beneficial. These included the use of 3% acetic acid in ethanol (or ...

journal_title：Mutagenesis

authors： Amphlett NW,Mitchell ID,Rees RW,Haynes GA

更新日期：1996-01-01 00:00:00

abstract：:The genotoxicity of alpha-hexachlorocyclohexane (alpha-HCH) was evaluated in primary cultures of mouse, rat and human hepatocytes. DNA fragmentation was measured by the alkaline elution technique and DNA repair synthesis by quantitative autoradiography. A 20 h exposure to subtoxic concentrations ranging from 0.056 to ...

journal_title：Mutagenesis

authors： Mattioli F,Robbiano L,Adamo D,Federa R,Martelli A,Brambilla G

更新日期：1996-01-01 00:00:00

abstract：:The presence of centromeric DNA was studied in micronuclei isolated from the blood of male ddY mice after five weekly intraperitoneal injections of mitomycin C (MMC), 1-beta-D-arabinofuranosylcytosine (Ara-C), colchicine (COL) or vinblastine sulfate (VBL). In agreement with our earlier findings, about half of the micr...

journal_title：Mutagenesis

authors： Mäki-Paakkanen J,Hayashi M,Suzuki T,Tanabe H,Honma M,Sofuni T

更新日期：1995-11-01 00:00:00